Comparative Study of Ki Etic Parameters of Bacterial a D Fu Gal Amylases

Bacterial amylase from Bacillus subtilis and fungal amylase from Aspergillus niger were potential source for industrial exploitation in the field of amylase production. Hence, this study on bacterial amylase and fungal amylase was done to compare the kinetic parameter of both the types of amylases. 30-60% ammonium sulfate precipitation has done which showed 90 % of purification of both the partially purified bacterial and fungal amylases. Dialysis was done which showed 98% of purification of the types of amylases (bacterial and fungal amylase). pH optima were 8.2 for bacterial amylase and 6.6 for fungal amylase. Optimum temperature was 50oC for bacterial amylase and 30oC for fungal amylase. Optimum time of incubation for maximal activity was 30 minutes for bacterial amylase and 50 minutes for fungal amylase. 10% of NaCl was found to be good activator which enhanced both the bacterial and fungal amylases. It was showed that bacterial amylase is more stable at alkaline pH and thermostable as compared to fungal amylase.